Nanomaterials for Sample Preparation
The heat-shrinking process leads to a hierarchical structure of microscale polyolefin folds layered with nanoscale silica lamella that can be fine-tuned via oxide deposition thickness. SEM analysis was performed on Nanobind with: a) 2 nm, b) 20 nm, c) 50 nm, d) 100 nm, e) 150 nm, and f) 200 nm of deposited silicon dioxide.
Yi invented NanoBind Technology, a liquid/solid two-phase DNA extraction technology capable of isolating high-purity DNA molecules with ultrahigh molecular weight that are ideally suited for single-molecule long-read sequencing based on shrink-induced hierarchical silica nanofilm. This technology was successfully commercialized by Circulomics Inc. which was acquired by Pacific Biosciences. Nanobind has already become the standard sample preparing technology for third-generation DNA sequencing platforms.
A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction, Advanced Materials, 2016, 28, 10630–10636
Nanomaterials for Biosensing
SEM images, TEM images (upper left inset), geometric models (upper right inset), and ultravioletvisible (UV–vis) spectrum (lower inset) of A) Au NPs, B) Au@Ag coreshell NPs, and C) Au/Ag hollow NPs. D) Vials containing Au/Ag NPs prepared by reacting Au@Ag core-shell NP solution with different volumes of a 20 mm HAuCl4 solution.
Color combinations using NPs of three primary colors. A) Combination of NPs of three primary colors results in NP mixtures of unique secondary colors. B) Color palette by mixing primary NPs at various ratios. Droplets in the first row are mixtures of yellow and magenta NPs, droplets in the second row are mixtures of yellow and cyan NPs, and droplets in the third row are mixtures of magenta and cyan NPs. The ratios of the two NPs in the droplet from left to right are 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, and 0:10, respectively. C) FITC-labeled dsDNA target detected by single primary NPs and mixture of NPs.
Two-parameter multiplexing based on spatial separation and color co-localization. A) Detection schematics. Cyan, magenta, and yellow NPs are represented by spheres of their respective colors. B) Multiplexed detection of all 32 possible target combinations. Target combinations were reported by the location and the color of the test spot. Co-localization of multiple primary NPs resulted in unique secondary colors.
Multi-Color Au/Ag Nanoparticles for Multiplexed Lateral Flow Assay Based on Spatial Separation and Color Co-Localization, Advanced Functional Materials, 2021, 2109553.